ABSTRACT
Objective:To investigate the diagnostic value of combined detection of urine α 1-microglobulin(α 1-mG), N-acetyl-β-D-glucosaminidase (NAG), retinol binding protein (RBP) and β 2-microglobulin (β 2-mG) for early hypertensive kidney injury. Methods:From June 2016 to December 2018, 116 hypertension patients with renal damage (HRD group) and 44 cases with simple hypertension(HBP group) were selected in the Central People′s Hospital of Tengzhou in this study.And 36 cases of healthy people during the same period were selected as the control group.One hundred and sixteen cases of the HRD group were divided into Ⅰ-Ⅱ group (61 cases) and Ⅲ-Ⅴ group (55 cases) according to the classification of chronic kidney disease(CKD). The concentrations of α 1-mG, NAG, RBP and β 2-mG in urine were detected in patients and healthy people respectively.SPSS 19.0 software was used to perform statistical analysis. Results:The concentrations of urine α 1-mG, NAG, RBP, β 2-mG in the HRD group were (41.77±24.21)mg/L, (22.60±13.24)U/L, (2.86±1.73)mg/L, (1.76±0.95)mg/L, respectively, which in the HBP group were (12.49±8.10)mg/L, (13.45±8.61)U/L, (0.31±0.16)mg/L, (0.38±0.38)mg/L, respectively, which in the control group were (4.37±2.52)mg/L, (6.12±3.57)U/L, (0.29±0.17)mg/L, (0.28±0.15)mg/L, respectively.The concentrations of urine α 1-mG, NAG, RBP, β 2-mG in the HRD group were significantly higher than those in the HBP group( t=4.07, 4.25, 4.09, 4.03, all P<0.05) and the control group( t=3.15, 4.94, 2.49, 2.61, all P<0.05). The urine levels of α 1-mG, NAG, RBP, β 2-mG in phase Ⅰ-Ⅱ group were (21.62±13.45)mg/L, (21.96±12.49)U/L, (0.5±0.47)mg/L, (0.93±0.62)mg/L, respectively, which in the phase Ⅲ-Ⅴ group were (64.11±60.12)mg/L, (23.32±14.11)U/L, (5.48±4.77)mg/L, (2.68±2.55)mg/L, respectively.The concentrations of urine α 1-mG, NAG, RBP and β 2-mG in Ⅰ-Ⅱ group ( t=5.08, 4.99, 2.96, 1.66, all P<0.05) and Ⅲ-Ⅴ group ( t=3.95, 4.81, 4.33, 3.74, all P<0.05) were significantly higher than those in the control group.The levels of α 1-mG, RBP and β 2-mG in group Ⅲ-Ⅴ were higher than those in group Ⅰ-Ⅱ( t=5.37, 8.11, 4.52, all P<0.05). The positive detection rates of α 1-mG, NAG, RBP, β 2-mG and combination test in phase Ⅰ-Ⅱ group were 70.5%, 77.0%, 19.7%, 60.7%, 91.8%, respectively, which in the phase Ⅲ-Ⅴ group were 81.8%, 81.8%, 69.1%, 69.1% and 96.4%, respectively.The positive rate of urine α 1-mG, NAG, RBP and β 2-mG combination test was significantly higher than that of the single detection (phase Ⅰ-Ⅱ group: χ 2=7.71, 3.99, 61.4, 14.65; phase Ⅲ-Ⅴ group: χ 2=4.58, 4.58, 12.47, 12.47; all P<0.05). Conclusion:Urine α 1-mG, NAG, RBP and β 2-mG are important biochemical indicators in patients with early hypertensive kidney injury.The combined detection of the four tests has high diagnostic value in the diagnosis of early hypertensive nephropathy.
ABSTRACT
Objective To investigate the clinical value of serum alpha fetoprotein -L3 ( AFP-L3) and Golgi body protein (GP73) in the diagnosis and assessment of primary liver cancer.Methods From October 2013 to May 2017,125 cases with primary liver cancer in Tengzhou Central People's Hospital were selected as hepatocellular carcinoma(HCC) group,and 60 patients with benign liver lesions were selected as benign lesion group ,in the same period,60 healthy people were selected as control group.The separation of AFP -L3 was through microspincolum method,enzyme-linked immunosorbent assay was used to detect GP 73.Results The serum AFP -L3 and GP73 levels in the HCC group were (33.16 ±9.17)%,(309.61 ±10.16) μg/L,respectively,which in the benign lesion group were (4.19 ±1.97)%,(43.18 ±9.17) μg/L,respectively,which in the control group were (3.25 ± 1.26)%,(38.52 ±7.09)μg/L,respectively.The serum levels of AFP-L3 and GP73 in the HCC group were signif-icantly higher than those in benign lesion group and control group (t ＝13.264,9.065,15.328,10.826,all P＜0.05).The serum levels of AFP-L3 and GP73 between the benign lesion group and control group had no statistically significant differences (t＝0.635,0.772,all P＞0.05).The sensitivity,specificity and accuracy of AFP -L3 in the diagnosis of primary liver cancer were 74.2%,87.5%and 91.2%,respectively.The sensitivity,specificity and accu-racy of GP73 in the diagnosis of primary liver cancer were 84.7%,81.4%and 92.4%,respectively.The sensitivity, specificity and accuracy of combined detection of them in diagnosis of primary liver cancer were 98.2%,89.6%and 97.1%,respectively.The sensitivity and accuracy of the combined tests were significantly higher than AFP -L3 or GP73 alone (t＝5.026,4.114,3.018,2.776,all P＜0.05).According to TNM staging,125 patients with HCC were divided into stage Ⅰ+Ⅱ and Ⅲ+Ⅳ.The serum AFP -L3 and GP73 levels in patients with stage Ⅰ+Ⅱ were (28.25 ±8.26)%,(202.86 ±9.24) μg/L,respectively,which were significantly lower than those in patients with stage Ⅲ+Ⅳ[(43.17 ±10.24)%,(398.62 ±11.35)μg/L] (t ＝3.772,3.182,all P ＜0.05).Conclusion The serum levels of AFP -L3 and GP73 in patients with primary liver cancer are obviously higher.Combined detection can improve the sensitivity and accuracy of diagnosis ,and the degree of increase is related to the severity of the patients.